LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.
For general laboratory use.
供一般实验室使用。
Product No:03539806001 产品编号:03539806001
Pack Size:100 U (100 reactions) 包装尺寸:100 U(100 次反应)
Keywords: Other qPCR Consumables 关键词:其他 qPCR 耗材
Overview概述:
LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.
LightCycler ® 尿嘧啶-DNA糖基化酶水解单链和双链DNA中U-DNA位点的尿嘧啶-糖苷键,切除尿嘧啶并在DNA中产生对碱和热敏感的脱碱基位点。
The enzyme is more active on single-stranded DNA than on double- stranded DNA.
该酶对单链 DNA 的活性高于对双链 DNA 的活性。
The enzyme is inactive on RNA and native, uracil-free DNA.
该酶对 RNA 和天然无尿嘧啶 DNA 无活性。
Contents 内容
The enzyme is supplied in storage buffer at 2 U/µl.
该酶以 2 U/µl 的浓度提供在储存缓冲液中。
Application应用:
LightCycler® Uracil-DNA Glycosylase (LightCycler® UNG) is ideally suited for preventing carryover contamination between PCRs. This technique involves incorporating deoxyuridine triphosphate (dUTP) into all amplification reactions, and pretreating all successive LightCycler® FastStart Enzyme-based PCR mixtures with LightCycler® UNG. Prior to the thermal-cycling procedure, LightCycler® UNG is added to the LightCycler® amplification reaction mixture, then incubated for 10 minutes at +40°C. During the initial activation of FastStart DNA Polymerase (+95°C for 10 minutes), the LightCycler® UNG becomes inactive.
LightCycler ® 尿嘧啶-DNA 糖基化酶 (LightCycler ® UNG) 非常适合防止 PCR 之间的残留污染。该技术包括将脱氧尿苷三磷酸 (dUTP) 加入所有扩增反应中,并使用 LightCycler ® UNG预处理所有连续的基于LightCycler ® FastStart 酶的 PCR 混合物。在热循环程序之前,将 LightCycler ® UNG 添加到 LightCycler ® 扩增反应混合物中,然后在 +40°C 下孵育 10 分钟。在 FastStart DNA 聚合酶的初始激活期间(+95°C 10 分钟),LightCycler ® UNG 变得不活跃。
Note: LightCycler® UNG is designed only for use with the FastStart Enzyme or FastStart Enzyme-based LightCycler® Kits.
注意: LightCycler® UNG 仅设计用于 FastStart 酶或基于 FastStart 酶的 LightCycler ® 试剂盒。
属性 | 参数 |
产品名称 | 规格型号 | 简介/参数 | 市场价 |
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罗氏试剂 Roche LightCycler® Uracil-DNA Glycosylase | 03539806001 | LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA. | 0.00 |
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