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罗氏试剂 Roche LightCycler® Uracil-DNA Glycosylase

罗氏试剂 Roche LightCycler® Uracil-DNA Glycosylase

03539806001
03539806001
品牌:
罗氏/Roche
市场价:¥0.00

LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.

For general laboratory use.
供一般实验室使用。
 
Product No:03539806001   产品编号:03539806001  
Pack Size:100 U (100 reactions)  包装尺寸:100 U(100 次反应)
 
Keywords: Other qPCR Consumables  关键词:其他 qPCR 耗材
 
 
Overview概述:
LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.
LightCycler ® 尿嘧啶-DNA糖基化酶水解单链和双链DNA中U-DNA位点的尿嘧啶-糖苷键,切除尿嘧啶并在DNA中产生对碱和热敏感的脱碱基位点。
The enzyme is more active on single-stranded DNA than on double- stranded DNA.
该酶对单链 DNA 的活性高于对双链 DNA 的活性。
The enzyme is inactive on RNA and native, uracil-free DNA.
该酶对 RNA 和天然无尿嘧啶 DNA 无活性。
Contents 内容
The enzyme is supplied in storage buffer at 2 U/µl.
该酶以 2 U/µl 的浓度提供在储存缓冲液中。
 
Application应用:
LightCycler® Uracil-DNA Glycosylase (LightCycler® UNG) is ideally suited for preventing carryover contamination between PCRs. This technique involves incorporating deoxyuridine triphosphate (dUTP) into all amplification reactions, and pretreating all successive LightCycler® FastStart Enzyme-based PCR mixtures with LightCycler® UNG. Prior to the thermal-cycling procedure, LightCycler® UNG is added to the LightCycler® amplification reaction mixture, then incubated for 10 minutes at +40°C. During the initial activation of FastStart DNA Polymerase (+95°C for 10 minutes), the LightCycler® UNG becomes inactive.
 
LightCycler ® 尿嘧啶-DNA 糖基化酶 (LightCycler ®  UNG) 非常适合防止 PCR 之间的残留污染。该技术包括将脱氧尿苷三磷酸 (dUTP) 加入所有扩增反应中,并使用 LightCycler ®  UNG预处理所有连续的基于LightCycler ®  FastStart 酶的 PCR 混合物。在热循环程序之前,将 LightCycler ®  UNG 添加到 LightCycler ® 扩增反应混合物中,然后在 +40°C 下孵育 10 分钟。在 FastStart DNA 聚合酶的初始激活期间(+95°C 10 分钟),LightCycler ®  UNG 变得不活跃。
 
Note: LightCycler® UNG is designed only for use with the FastStart Enzyme or FastStart Enzyme-based LightCycler® Kits.
注意: LightCycler®  UNG 仅设计用于 FastStart 酶或基于 FastStart 酶的 LightCycler ® 试剂盒。

 

属性 参数
产品名称 规格型号 简介/参数 市场价
罗氏试剂 Roche EvoScript Universal cDNA Master 07912374001 Easy-to-use reaction mix for cDNA synthesis for two-step RT-qPCR For general laboratory use. 0.00
罗氏试剂 Roche EvoScript RNA SYBR 07912498001 罗氏试剂 Roche EvoScript RNA SYBR Green I Master (ROX) The EvoScript RNA SYBR® Green I Master (ROX) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays on instruments requiring ROX dye. For general laboratory use. 0.00
罗氏试剂 Roche EvoScript RNA Probes Master 07912269001 The EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. EvoScript RNA Probes Master 专为灵敏、高 特异性、高精度的一步法 RT-qPCR 而设计,尤其适用于基因表达分析。 For general laboratory use. 供一般实验室使用。 0.00
罗氏/roche LightCycler® DNA Master HybProbe 12015102001 Easy-to-use fast high-performance qPCR kit for HybProbe signal transmission with all LightCycler® Carousel-Based Instruments. 易于使用的 qPCR 试剂盒,适用于所有基于LightCycler ® 转盘的仪器的HybProbe 信号传输。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche LightCycler® Fluorescein CPG 03138178001 3' Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments. 在所有 LightCycler ® 480 和 LightCycler ®转盘式 系统仪器上标记用于单色和多色检测的 HybProbe 探针时的 3' 染料。 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。 0.00
罗氏试剂 Roche RealTime ready Cell Lysis KitLightCycler® Red 610-N-hydroxysuccinimide ester 03561488001 Blue solid, for a minimum of 250 nmol oligonucleotides 蓝色固体,至少 250 nmol 寡核苷酸 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。  0.00
罗氏试剂 Roche LightCycler® Uracil-DNA Glycosylase 03539806001 LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA. 0.00
罗氏试剂 Roche LightCycler ® Multiplex DNA Master 07339577001 Easy-to-use 5x reaction mix optimized for multiplex qPCR, compatible with the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems. 易于使用的5倍反应混合优化的多重qPCR,与LightCycler®480或LightCycler®96实时PCR系统兼容。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche LightCycler® FastStart DNA Master HybProbe 03003248001 Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System. 易于使用的热启动反应混合物,用于使用 LightCycler ® 基于旋转木马的系统进行 PCR 。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche RealTime ready Cell Lysis Kit 06366821001 Reagent for lysing cells prior to two-step real-time RT-PCR applications. For life science research only. Not for use in diagnostic procedures. 0.00
罗氏试剂 Roche LightCycler®EvoScript RNA SYBR®Green I Master The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. LightCycler ® EvoScript RNA SYBR ® Green I Master专 为敏感、高 特异性、高精度一步RT-qPCR而设计,特别适用于基因表达分析。 0.00

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