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罗氏试剂 Roche LightCycler® Fluorescein CPG

罗氏试剂 Roche LightCycler® Fluorescein CPG

03138178001
03138178001
品牌:
罗氏/Roche
市场价:¥0.00

3' Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments.
在所有 LightCycler ® 480 和 LightCycler ®转盘式 系统仪器上标记用于单色和多色检测的 HybProbe 探针时的 3' 染料。
For life science research only. Not for use in diagnostic procedures.
仅用于生命科学研究。不用于诊断过程。

Product No:03138178001   产品编号:03138178001  
Pack Size:1g   包装尺寸:1克
 
Keywords: Other qPCR Consumables  关键词:其他 qPCR 耗材
 
Overview概述:
 
Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3' terminus of oligonucleotides.
用于标记寡核苷酸 3' 末端的荧光素长链烷基氨基控制孔玻璃(lcaa CPG,500 Å)。
 
Contents 内容
white solid
白色固体
 
Application应用:
LightCycler® Fluorescein CPG labels the 3' end of an oligonucleotide that is combined with a 5' LightCycler® Red-laeled oligonucleotide when using HybProbe probes for detection. This support can be used in the same manner as standard nucleoside supports since it contains a dimethobxytrityl group.
 
当使用 HybProbe 探针进行检测时,LightCycler ® 荧光素 CPG 标记寡核苷酸的 3' 末端,该寡核苷酸与 5' LightCycler ®  Red-laeled 寡核苷酸结合。该支持物可以以与标准核苷支持物相同的方式使用,因为它含有二甲氧基三苯甲基。

Detail 细节
 
The LightCycler® Fluorescein CPG is derived from a pure dye isomer, and is attached via a linkage that is stable during oligonucleotide synthesis. Therefore, HPLC purification is simple and results in a well-defined product. HybProbe probes synthesized using the LightCycler® Fluorescein CPG are blocked at the 3' terminus, which prevents them from Taq DNA polymerase extension and exonuclease digestion. Thus, the resulting oligonucleotide is perfectly suited for mono- and dual-color experiments using the LightCycler® Carousel-Based System Instruments.
 
LightCycler ® 荧光素 CPG 源自纯染料异构体,并通过在寡核苷酸合成过程中稳定的连接键连接。因此,HPLC 纯化很简单,可以得到明确定义的产品。使用 LightCycler ® 荧光素 CPG合成的 HybProbe 探针在 3' 末端被封闭,从而阻止它们进行 Taq DNA 聚合酶延伸和核酸外切酶消化。因此,所得寡核苷酸非常适合使用 LightCycler ® 基于旋转木马的系统仪器进行单色和双色实验。
 
Spectral characteristics for an oligonucleotide labeled with LightCycler® Fluorescein:
Excitation maximum: 494 nm
Emission maximum: 519 nm (both measured in 2 mM Tris buffer, pH 8.3).
 
用 LightCycler ® 荧光素标记的寡核苷酸的光谱特征:
最大激发波长:494 nm
发射最大值:519 nm(均在 2 mM Tris 缓冲液,pH 8.3 中测量)。

 

属性 参数
产品名称 规格型号 简介/参数 市场价
罗氏试剂 Roche EvoScript Universal cDNA Master 07912374001 Easy-to-use reaction mix for cDNA synthesis for two-step RT-qPCR For general laboratory use. 0.00
罗氏试剂 Roche EvoScript RNA SYBR 07912498001 罗氏试剂 Roche EvoScript RNA SYBR Green I Master (ROX) The EvoScript RNA SYBR® Green I Master (ROX) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays on instruments requiring ROX dye. For general laboratory use. 0.00
罗氏试剂 Roche EvoScript RNA Probes Master 07912269001 The EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. EvoScript RNA Probes Master 专为灵敏、高 特异性、高精度的一步法 RT-qPCR 而设计,尤其适用于基因表达分析。 For general laboratory use. 供一般实验室使用。 0.00
罗氏/roche LightCycler® DNA Master HybProbe 12015102001 Easy-to-use fast high-performance qPCR kit for HybProbe signal transmission with all LightCycler® Carousel-Based Instruments. 易于使用的 qPCR 试剂盒,适用于所有基于LightCycler ® 转盘的仪器的HybProbe 信号传输。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche LightCycler® Fluorescein CPG 03138178001 3' Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments. 在所有 LightCycler ® 480 和 LightCycler ®转盘式 系统仪器上标记用于单色和多色检测的 HybProbe 探针时的 3' 染料。 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。 0.00
罗氏试剂 Roche RealTime ready Cell Lysis KitLightCycler® Red 610-N-hydroxysuccinimide ester 03561488001 Blue solid, for a minimum of 250 nmol oligonucleotides 蓝色固体,至少 250 nmol 寡核苷酸 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。  0.00
罗氏试剂 Roche LightCycler® Uracil-DNA Glycosylase 03539806001 LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA. 0.00
罗氏试剂 Roche LightCycler ® Multiplex DNA Master 07339577001 Easy-to-use 5x reaction mix optimized for multiplex qPCR, compatible with the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems. 易于使用的5倍反应混合优化的多重qPCR,与LightCycler®480或LightCycler®96实时PCR系统兼容。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche LightCycler® FastStart DNA Master HybProbe 03003248001 Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System. 易于使用的热启动反应混合物,用于使用 LightCycler ® 基于旋转木马的系统进行 PCR 。 For general laboratory use. 供一般实验室使用。 0.00
罗氏试剂 Roche RealTime ready Cell Lysis Kit 06366821001 Reagent for lysing cells prior to two-step real-time RT-PCR applications. For life science research only. Not for use in diagnostic procedures. 0.00
罗氏试剂 Roche LightCycler®EvoScript RNA SYBR®Green I Master The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. LightCycler ® EvoScript RNA SYBR ® Green I Master专 为敏感、高 特异性、高精度一步RT-qPCR而设计,特别适用于基因表达分析。 0.00

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