3' Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments.
在所有 LightCycler ® 480 和 LightCycler ®转盘式 系统仪器上标记用于单色和多色检测的 HybProbe 探针时的 3' 染料。
For life science research only. Not for use in diagnostic procedures.
仅用于生命科学研究。不用于诊断过程。
Product No:03138178001 产品编号:03138178001
Pack Size:1g 包装尺寸:1克
Keywords: Other qPCR Consumables 关键词:其他 qPCR 耗材
Overview概述:
Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3' terminus of oligonucleotides.
用于标记寡核苷酸 3' 末端的荧光素长链烷基氨基控制孔玻璃(lcaa CPG,500 Å)。
Contents 内容
white solid
白色固体
Application应用:
LightCycler® Fluorescein CPG labels the 3' end of an oligonucleotide that is combined with a 5' LightCycler® Red-laeled oligonucleotide when using HybProbe probes for detection. This support can be used in the same manner as standard nucleoside supports since it contains a dimethobxytrityl group.
当使用 HybProbe 探针进行检测时,LightCycler ® 荧光素 CPG 标记寡核苷酸的 3' 末端,该寡核苷酸与 5' LightCycler ® Red-laeled 寡核苷酸结合。该支持物可以以与标准核苷支持物相同的方式使用,因为它含有二甲氧基三苯甲基。
Detail 细节
The LightCycler® Fluorescein CPG is derived from a pure dye isomer, and is attached via a linkage that is stable during oligonucleotide synthesis. Therefore, HPLC purification is simple and results in a well-defined product. HybProbe probes synthesized using the LightCycler® Fluorescein CPG are blocked at the 3' terminus, which prevents them from Taq DNA polymerase extension and exonuclease digestion. Thus, the resulting oligonucleotide is perfectly suited for mono- and dual-color experiments using the LightCycler® Carousel-Based System Instruments.
LightCycler ® 荧光素 CPG 源自纯染料异构体,并通过在寡核苷酸合成过程中稳定的连接键连接。因此,HPLC 纯化很简单,可以得到明确定义的产品。使用 LightCycler ® 荧光素 CPG合成的 HybProbe 探针在 3' 末端被封闭,从而阻止它们进行 Taq DNA 聚合酶延伸和核酸外切酶消化。因此,所得寡核苷酸非常适合使用 LightCycler ® 基于旋转木马的系统仪器进行单色和双色实验。
Spectral characteristics for an oligonucleotide labeled with LightCycler® Fluorescein:
Excitation maximum: 494 nm
Emission maximum: 519 nm (both measured in 2 mM Tris buffer, pH 8.3).
用 LightCycler ® 荧光素标记的寡核苷酸的光谱特征:
最大激发波长:494 nm
发射最大值:519 nm(均在 2 mM Tris 缓冲液,pH 8.3 中测量)。
属性 | 参数 |
产品名称 | 规格型号 | 简介/参数 | 市场价 |
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罗氏试剂 Roche EvoScript Universal cDNA Master | 07912374001 | Easy-to-use reaction mix for cDNA synthesis for two-step RT-qPCR For general laboratory use. | 0.00 |
罗氏试剂 Roche EvoScript RNA SYBR | 07912498001 | 罗氏试剂 Roche EvoScript RNA SYBR Green I Master (ROX) The EvoScript RNA SYBR® Green I Master (ROX) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays on instruments requiring ROX dye. For general laboratory use. | 0.00 |
罗氏试剂 Roche EvoScript RNA Probes Master | 07912269001 | The EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. EvoScript RNA Probes Master 专为灵敏、高 特异性、高精度的一步法 RT-qPCR 而设计,尤其适用于基因表达分析。 For general laboratory use. 供一般实验室使用。 | 0.00 |
罗氏/roche LightCycler® DNA Master HybProbe | 12015102001 | Easy-to-use fast high-performance qPCR kit for HybProbe signal transmission with all LightCycler® Carousel-Based Instruments. 易于使用的 qPCR 试剂盒,适用于所有基于LightCycler ® 转盘的仪器的HybProbe 信号传输。 For general laboratory use. 供一般实验室使用。 | 0.00 |
罗氏试剂 Roche LightCycler® Fluorescein CPG | 03138178001 | 3' Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments. 在所有 LightCycler ® 480 和 LightCycler ®转盘式 系统仪器上标记用于单色和多色检测的 HybProbe 探针时的 3' 染料。 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。 | 0.00 |
罗氏试剂 Roche RealTime ready Cell Lysis KitLightCycler® Red 610-N-hydroxysuccinimide ester | 03561488001 | Blue solid, for a minimum of 250 nmol oligonucleotides 蓝色固体,至少 250 nmol 寡核苷酸 For life science research only. Not for use in diagnostic procedures. 仅用于生命科学研究。不用于诊断过程。 | 0.00 |
罗氏试剂 Roche LightCycler® Uracil-DNA Glycosylase | 03539806001 | LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA. | 0.00 |
罗氏试剂 Roche LightCycler ® Multiplex DNA Master | 07339577001 | Easy-to-use 5x reaction mix optimized for multiplex qPCR, compatible with the LightCycler® 480 or the LightCycler® 96 Real-Time PCR Systems. 易于使用的5倍反应混合优化的多重qPCR,与LightCycler®480或LightCycler®96实时PCR系统兼容。 For general laboratory use. 供一般实验室使用。 | 0.00 |
罗氏试剂 Roche LightCycler® FastStart DNA Master HybProbe | 03003248001 | Easy-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System. 易于使用的热启动反应混合物,用于使用 LightCycler ® 基于旋转木马的系统进行 PCR 。 For general laboratory use. 供一般实验室使用。 | 0.00 |
罗氏试剂 Roche RealTime ready Cell Lysis Kit | 06366821001 | Reagent for lysing cells prior to two-step real-time RT-PCR applications. For life science research only. Not for use in diagnostic procedures. | 0.00 |
罗氏试剂 Roche LightCycler®EvoScript RNA SYBR®Green I Master | The LightCycler® EvoScript RNA SYBR® Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. LightCycler ® EvoScript RNA SYBR ® Green I Master专 为敏感、高 特异性、高精度一步RT-qPCR而设计,特别适用于基因表达分析。 | 0.00 |